The adrenaline test for enzymes is a colorimetric enzyme assay based on the quantification of periodate-sensitive reaction products such as 1,2-diols and 1,2-aminoalcohols by back-titration of the ...
This article shows how enzymatic activity can be effectively determined by means of the Eppendorf BioSpectrometer. The preliminary measurements were carried out by utilizing the “single λ-continuous” ...
Temperature and substrate availability constrain the activity of the extracellular enzymes that decompose and release nutrients from soil organic matter (SOM). Proteolytic enzymes are the primary ...
Note: this practical uses the temperature of 35°C. This is close to body temperature. Warmer temperatures may denature the enzymes and the reaction will take longer at lower temperatures.
The speed at which an enzyme catalyzes a reaction typically doubles with every 10 °C increase in temperature. But analogous enzymes found in tropical fish and Arctic fish tend to work at the same rate ...
Scientists studying a COVID-19 coronavirus enzyme at temperatures ranging from frosty to human-body warm discovered subtle structural shifts that offer clues about how the enzyme works. The findings ...
This accurate thermosensation relies on the precise maintenance of thermal receptor functions; however, their activation and regulatory mechanisms have remained unclear. In a new study, researchers ...