The adrenaline test for enzymes is a colorimetric enzyme assay based on the quantification of periodate-sensitive reaction products such as 1,2-diols and 1,2-aminoalcohols by back-titration of the ...
Using Eppendorf BioSpectrometer for the determination of enzyme activity and substrate concentration
This article shows how enzymatic activity can be effectively determined by means of the Eppendorf BioSpectrometer. The preliminary measurements were carried out by utilizing the “single λ-continuous” ...
Temperature and substrate availability constrain the activity of the extracellular enzymes that decompose and release nutrients from soil organic matter (SOM). Proteolytic enzymes are the primary ...
Note: this practical uses the temperature of 35°C. This is close to body temperature. Warmer temperatures may denature the enzymes and the reaction will take longer at lower temperatures.
The speed at which an enzyme catalyzes a reaction typically doubles with every 10 °C increase in temperature. But analogous enzymes found in tropical fish and Arctic fish tend to work at the same rate ...
Scientists studying a COVID-19 coronavirus enzyme at temperatures ranging from frosty to human-body warm discovered subtle structural shifts that offer clues about how the enzyme works. The findings ...
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Scientists discover lipid enzyme for maintaining cool temperature sensation and avoidance in fruit fly larvae
This accurate thermosensation relies on the precise maintenance of thermal receptor functions; however, their activation and regulatory mechanisms have remained unclear. In a new study, researchers ...
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