Creating analytical tools that support the advancement of knowledge can often come about through the integration of existing information and techniques, theoretical and practical, directed toward a ...

Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

Today, the ability to obtain pure proteins is essential for developing targeted drugs, creating vaccines and understanding biological processes at a molecular level. The development and optimization ...