In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...

Most restriction endonucleases use Mg²⁺ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that Bfil, a metal-independent restriction enzyme from the phospholipase D superfamily, ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

This is a preview. Log in through your library . Abstract Here we report the cloning and preliminary characterization of the Pst I restriction-modification system of Providencia stuartii 164.

WEST LAFAYETTE, Ind. – Researchers at Purdue University have attached magnetic "nanoparticles" to DNA and then cut these "DNA wires" into pieces, offering the promise of creating low-cost, ...