Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...
Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...
Premier Biosoft’s AlleleID Was Designed to Assist in Development of Diagnostic Assays Efficient clinical diagnosis of pathogens is important for the management of infectious diseases. Conventional ...
COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, v2.0, by Roche Molecular Diagnostics The FDA has approved the COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, v2.0, a dual-PCR target HIV-1 viral load test from Roche ...
Courtesy of Applied Biosystems THE TAQMAN PROCEDURE: Applied Biosystems' TaqMan procedure relies on the 5'-to-3' exonuclease activity of Taq polymerase. The TaqMan probe bears a fluoro-phore at the 5' ...
Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages over ...
This is a preview. Log in through your library . Abstract Real-time polymerase chain reaction (real-time PCR), also known as quantitative PCR, is used to determine relative gene expression or to ...
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