Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

While Western blots appear straightforward, there is room for optimization at every step of the process to transform blurry blots into crisp, reliable results. In this Technique Talk sponsored by ...

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...